8T48
The N4BP1 CUE-like domain in complex with linear di-Ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.789, 118.716, 50.695 |
| Unit cell angles | 90.00, 91.72, 90.00 |
Refinement procedure
| Resolution | 46.600 - 2.000 |
| R-factor | 0.1629 |
| Rwork | 0.160 |
| R-free | 0.21780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.864 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 2.072 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.087 | 0.476 |
| Number of reflections | 28171 | 2751 |
| <I/σ(I)> | 11.52 | |
| Completeness [%] | 97.2 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Tris pH 8.5, 30% (w/v) PEG 4K, 0.2 M Li2SO4 |
