8T3J
Crystal structure of native exfoliative toxin C (ExhC) from Mammaliicoccus sciuri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS SIRIUS BEAMLINE MANACA |
| Synchrotron site | LNLS SIRIUS |
| Beamline | MANACA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-03 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.977180 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 167.094, 167.094, 157.743 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.283 - 2.705 |
| Rwork | 0.211 |
| R-free | 0.27010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.546 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.283 | 2.807 |
| High resolution limit [Å] | 2.705 | 2.710 |
| Rpim | 0.067 | 0.752 |
| Number of reflections | 30672 | 3017 |
| <I/σ(I)> | 8.4 | 0.79 |
| Completeness [%] | 99.9 | |
| Redundancy | 26.2 | |
| CC(1/2) | 0.995 | 0.450 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M HEPES pH 7.5 and 0.8 M Potassium/Sodium tartrate |
