8T33
Crystal structure of K46 acetylated GABARAP in complex with the LIR of TP53INP2/DOR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.522 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 53.488, 89.178, 63.967 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.870 - 1.599 |
R-factor | 0.1874 |
Rwork | 0.185 |
R-free | 0.21230 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.870 | 1.657 |
High resolution limit [Å] | 1.599 | 1.599 |
Number of reflections | 39016 | 3866 |
<I/σ(I)> | 23.94 | |
Completeness [%] | 99.8 | |
Redundancy | 4.4 | |
CC(1/2) | 0.999 | 0.961 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10 % w/v PEG 3000, 0.2 M Zinc acetate dihydrate, 0.1 M Sodium acetate PH 4.5 |