8T2J
Structure of the catalytic domain of PPM1D/Wip1 serine/threonine phosphatase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.664, 59.930, 49.566 |
Unit cell angles | 90.00, 92.13, 90.00 |
Refinement procedure
Resolution | 25.650 - 1.800 |
R-factor | 0.1764 |
Rwork | 0.175 |
R-free | 0.21920 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.316 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 8.050 | 1.850 |
High resolution limit [Å] | 1.800 | 5.690 | 1.800 |
Rmerge | 0.066 | 0.053 | 0.491 |
Rmeas | 0.071 | 0.058 | 0.532 |
Number of reflections | 26792 | 557 | 2010 |
<I/σ(I)> | 15.75 | ||
Completeness [%] | 98.9 | ||
Redundancy | 4.67 | ||
CC(1/2) | 0.998 | 0.996 | 0.911 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100 mM HEPES pH 7.5, 10% (w/v) PEG 3000, 20% PEG 400 (v/v), and 10% glycerol (v/v) |