8T1R

Crystal structure of human CPSF73 catalytic segment in complex with compound 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	58.803, 82.311, 102.784
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.250 - 2.200
R-factor	0.1986
Rwork	0.196
R-free	0.24990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.518
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.250	2.330
High resolution limit [Å]	2.200	2.200
Number of reflections	25871	4087
<I/σ(I)>	10.2	2
Completeness [%]	99.7	98.5
Redundancy	5.6	5.6
CC(1/2)	0.997	0.627

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	18-30% (w/v) PEG3350, 200-250 mM ammonium sulfate and 0.1 M Bis-Tris pH 6.3-6.7