8SZ5
[2T5] Self-assembling DNA motif with 5 base pairs between junctions and P32 symmetry
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-24 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 1.00743 |
Spacegroup name | P 32 |
Unit cell lengths | 69.089, 69.089, 59.208 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.540 - 2.890 |
R-factor | 0.2015 |
Rwork | 0.198 |
R-free | 0.25640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6x8c |
RMSD bond length | 0.004 |
RMSD bond angle | 0.583 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 59.833 | 59.833 | 3.169 |
High resolution limit [Å] | 2.890 | 9.450 | 2.892 |
Number of reflections | 3687 | 205 | 203 |
<I/σ(I)> | 8 | ||
Completeness [%] | 81.5 | ||
Redundancy | 11 | 9.7 | 9.5 |
CC(1/2) | 0.997 | 0.997 | 0.085 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 100 mM MOPS, 1.25 M magnesium sulfate |