8SXQ

Crystal structure of Sel-1 repeat protein LceB from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.18082
Spacegroup name	P 32
Unit cell lengths	116.790, 116.790, 88.350
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.720 - 2.700
R-factor	0.219
Rwork	0.217
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	13.702
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.720	2.797
High resolution limit [Å]	2.700	2.700
Rmerge	0.021
Number of reflections	36977	3696
<I/σ(I)>	20.5
Completeness [%]	99.9
Redundancy	4.7
CC(1/2)	0.999	0.499

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293.15	7mg/mL 0.1M Citric Acid pH 4.0 10% MPD