8SWZ

PARP4 ART domain bound to EB47

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.12
Spacegroup name	C 2 2 21
Unit cell lengths	116.848, 150.859, 104.613
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.240 - 3.000
R-factor	0.2058
Rwork	0.203
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.559
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.240	3.180
High resolution limit [Å]	3.000	3.000
Rmerge	0.114	2.022
Rmeas	0.118	2.096
Rpim	0.031	0.547
Total number of observations		43823
Number of reflections	35783	3020
<I/σ(I)>	18.2	1.4
Completeness [%]	100.0
Redundancy	14.5	14.5
CC(1/2)	0.999	0.521

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG3350, 0.2M sodium citrate tribasic dihydrate, 800uM EB47