8SWO
GpppA dinucleotide ligand binding to RNA UC template
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2022-10-15 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 44.121, 44.121, 84.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.190 - 1.640 |
| R-factor | 0.24945 |
| Rwork | 0.248 |
| R-free | 0.27450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hbx |
| RMSD bond length | 0.023 |
| RMSD bond angle | 3.845 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.700 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.136 | 0.796 |
| Number of reflections | 10994 | 1205 |
| <I/σ(I)> | 10.8 | 8.1 |
| Completeness [%] | 90.1 | 100 |
| Redundancy | 10.1 | 10.4 |
| CC(1/2) | 0.976 | 0.985 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.02 M magnesium chloride hexahydrate, 0.05 M MOPS, pH 7.0, 55% v/v Tacsimate, pH 7.0, 2 mM hexammine cobalt(III) chloride |
