8SWC

RNase H complex with ASO (OOO) and RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97856
Spacegroup name	P 31 2 1
Unit cell lengths	100.617, 100.617, 82.092
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.480 - 2.680
R-factor	0.193
Rwork	0.192
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qk9
RMSD bond length	0.003
RMSD bond angle	0.649
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.570	2.800
High resolution limit [Å]	2.670	2.670
Rmerge	0.070
Rmeas	0.072
Rpim	0.016
Number of reflections	13859	1677
<I/σ(I)>	30.2
Completeness [%]	98.9
Redundancy	19.7
CC(1/2)	1.000	0.871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	2:1 complex : precipitant (0.1 M sodium acetate, pH 4.6, 8% w/v PEG4000), cryoprotection: mother liquor + 25% v/v glycerol, 1 minute