8SWC
RNase H complex with ASO (OOO) and RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 100.617, 100.617, 82.092 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.480 - 2.680 |
| R-factor | 0.193 |
| Rwork | 0.192 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qk9 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.649 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.570 | 2.800 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmerge | 0.070 | |
| Rmeas | 0.072 | |
| Rpim | 0.016 | |
| Number of reflections | 13859 | 1677 |
| <I/σ(I)> | 30.2 | |
| Completeness [%] | 98.9 | |
| Redundancy | 19.7 | |
| CC(1/2) | 1.000 | 0.871 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 2:1 complex : precipitant (0.1 M sodium acetate, pH 4.6, 8% w/v PEG4000), cryoprotection: mother liquor + 25% v/v glycerol, 1 minute |
