8SWB

RNase H complex with streopure ASO and RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	46.545, 52.939, 106.008
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.620 - 2.000
R-factor	0.1856
Rwork	0.184
R-free	0.22380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qk9
RMSD bond length	0.008
RMSD bond angle	0.986
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.360	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.145
Rmeas	0.154
Rpim	0.051	0.795
Number of reflections	18366	1340
<I/σ(I)>	9
Completeness [%]	99.9
Redundancy	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	1:1 complex : precipitant (0.8 M ammonium sulfate, 0.1 M citrate, pH 4.0), cryoprotection: mother liquor + 1.75 M ammonium sulfate, 1 minute