8SVY

MBP-Mcl1 in complex with ligand 10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-15
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	99.040, 137.032, 38.715
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.280 - 1.470
R-factor	0.17176
Rwork	0.170
R-free	0.20633
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.421
Data reduction software	autoPROC (1.1.7)
Data scaling software	autoPROC ((Version 1.1.7))
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.280	1.490
High resolution limit [Å]	1.470	1.470
Rmeas	0.054	1.360
Rpim	0.021	0.527
Number of reflections	89536	4501
<I/σ(I)>	16.6	1.4
Completeness [%]	98.4	99.5
Redundancy	6.5
CC(1/2)	0.999	0.563

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	285	19% (w/v) PEG3350, 0.17M Mg formate