8SVC
Crystal Structure of SBP from Klebsiella pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-05 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.920099 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 62.187, 141.291, 71.388 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.918 - 1.590 |
R-factor | 0.1853 |
Rwork | 0.184 |
R-free | 0.21020 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.772 |
Data reduction software | autoPROC (1.0.5 (20211020)) |
Data scaling software | autoPROC |
Phasing software | PHENIX (1.13_2998) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.920 | 1.647 |
High resolution limit [Å] | 1.590 | 1.590 |
Rmerge | 0.053 | |
Rpim | 0.053 | |
Number of reflections | 42016 | 4014 |
<I/σ(I)> | 7.78 | |
Completeness [%] | 98.5 | |
Redundancy | 2 | |
CC(1/2) | 0.995 | 0.547 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M Ammonium Sulfate and 23% (w/v) PEG 4000 |