8SV6
Structure of the M. smegmatis DarR protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 94.422, 94.422, 215.055 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.210 - 3.560 |
R-factor | 0.2783 |
Rwork | 0.275 |
R-free | 0.30540 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.618 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.860 | 3.600 |
High resolution limit [Å] | 3.560 | 3.560 |
Rpim | 0.053 | 0.561 |
Number of reflections | 12397 | 1239 |
<I/σ(I)> | 7 | 1.5 |
Completeness [%] | 99.9 | |
Redundancy | 8 | |
CC(1/2) | 0.997 | 0.357 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 278 | 22% PEG 3350 and 0.1 M Citrate |