8SSU

ZnFs 3-11 of CCCTC-binding factor (CTCF) Complexed with 19mer DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 32 2 1
Unit cell lengths	80.464, 80.464, 187.598
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.340 - 2.890
R-factor	0.2018
Rwork	0.200
R-free	0.24030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.503
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.340	2.990
High resolution limit [Å]	2.890	2.890
Rpim	0.065	0.860
Number of reflections	30234	3041
<I/σ(I)>	12.8	1.5
Completeness [%]	99.9
Redundancy	10.3	9.7
CC(1/2)	0.974	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.04 M Citric acid, 0.06 M BIS-TRIS propane/pH 6.4, 20% w/v Polyethylene glycol 3,350