8SSJ
Room-temperature X-ray structure of human mitochondrial serine hydroxymethyltransferase (hSHMT2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2023-02-24 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 161.609, 161.609, 210.784 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.650 - 2.500 |
R-factor | 0.1737 |
Rwork | 0.172 |
R-free | 0.20460 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.522 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2-4158-000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rpim | 0.063 | 0.291 |
Number of reflections | 55062 | 5150 |
<I/σ(I)> | 10.5 | |
Completeness [%] | 97.0 | |
Redundancy | 6 | |
CC(1/2) | 0.980 | 0.765 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 287.15 | 0.1 M Tricine pH 8.4, 15% PEG 3350 |