8SSG

Minimal protein-only/RNA-free Ribonuclease P from Hydrogenobacter thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.03317
Spacegroup name	P 21 2 21
Unit cell lengths	102.317, 113.764, 155.306
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.739 - 3.200
Rwork	0.198
R-free	0.24850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7f3e
RMSD bond length	0.009
RMSD bond angle	2.076
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.840	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.116	1.799
Rmeas	0.137	2.104
Rpim	0.071	1.082
Number of reflections	30570	4394
<I/σ(I)>	12.1
Completeness [%]	99.9
Redundancy	6.8	7.2
CC(1/2)	0.999	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	277.15	1.0 M Sodium Malonate, pH 5.0, 0.1 M Sodium Acetate trihydrate, pH 4.5, 2% (w/v) polyethylene glycol 20,000