8SRZ
Structure of a bacterial death-like domain from Lysobacter enzymogenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2022-05-03 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 65 |
Unit cell lengths | 58.252, 58.252, 122.354 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.920 - 1.250 |
R-factor | 0.1591 |
Rwork | 0.159 |
R-free | 0.17760 |
Structure solution method | SAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.549 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX (1.19.1_4122) |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.640 | 1.270 |
High resolution limit [Å] | 1.250 | 1.250 |
Rpim | 0.014 | |
Number of reflections | 64141 | 64141 |
<I/σ(I)> | 23 | |
Completeness [%] | 99.0 | |
Redundancy | 17.3 | |
CC(1/2) | 1.000 | 0.881 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 4 M sodium formate |