8SP9
Crystal Structure of Coxsackievirus B3 (CVB3) Cloverleaf RNA with tRNA scaffold
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-15 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.429, 28.359, 113.111 |
Unit cell angles | 90.00, 92.01, 90.00 |
Refinement procedure
Resolution | 44.690 - 2.920 |
R-factor | 0.2917 |
Rwork | 0.291 |
R-free | 0.30820 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.688 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.700 | 2.970 |
High resolution limit [Å] | 2.900 | 2.920 |
Rpim | 0.094 | 0.401 |
Number of reflections | 9250 | 232 |
<I/σ(I)> | 5.74 | |
Completeness [%] | 83.3 | |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 100 mM Bis-Tris, pH 6.5, 48% polypropylene glycol (PPG)-P400 |