8SP5

LINE-1 retrotransposon endonuclease domain complex with Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92
Spacegroup name	P 21 21 2
Unit cell lengths	93.096, 122.378, 43.412
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.550 - 2.230
Rwork	0.203
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.888
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.550	2.300
High resolution limit [Å]	2.230	2.230
Number of reflections	25007	2244
<I/σ(I)>	9.2
Completeness [%]	100.0
Redundancy	13.4
CC(1/2)	0.996	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.14 M ammonium sulfate, 24% polyethylene glycol 5000 monomethyl ether, 5 mM magnesium chloride