8SP1

Chimeric ETS-domain of murine PU.1 harboring the corresponding beta-strand 3 (S3) residues from murine Ets-1 in complex with d(AATAAGCGIAAGTGGG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000000
Spacegroup name	P 1 21 1
Unit cell lengths	42.824, 60.725, 44.973
Unit cell angles	90.00, 117.27, 90.00

Refinement procedure

Resolution	33.390 - 1.620
R-factor	0.1686
Rwork	0.166
R-free	0.19930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.380
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.390	1.678
High resolution limit [Å]	1.620	1.620
Rmerge	0.048	0.414
Rmeas	0.057	0.492
Rpim	0.031	0.264
Number of reflections	25881	2541
<I/σ(I)>	14.63	2.92
Completeness [%]	98.9	97.25
Redundancy	3.4	3.3
CC(1/2)	0.998	0.958

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	100 mM Sodium Acetate, pH=4.6, 2% PEG 3350