8SOW

Structure of the complex formed by human interleukin-2 and scFv F10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	136.047, 40.215, 68.123
Unit cell angles	90.00, 111.21, 90.00

Refinement procedure

Resolution	38.450 - 1.710
Rwork	0.216
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.153
Data reduction software	autoPROC
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.450	1.790
High resolution limit [Å]	1.710	1.710
Rmerge	0.072	0.968
Number of reflections	37142	3657
<I/σ(I)>	12.68
Completeness [%]	98.7	98.33
Redundancy	6.9	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	15-30% PEG 3350, 0.1-0.35 M ammonium fluoride