8SNZ

X-ray Crystal Structure of FMN-bound long-chain flavodoxin from Rhodopseudomonas palustris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-03
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	46.664, 58.891, 60.904
Unit cell angles	90.00, 105.49, 90.00

Refinement procedure

Resolution	29.350 - 2.170
R-factor	0.2303
Rwork	0.229
R-free	0.25950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.391
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.350	2.248
High resolution limit [Å]	2.170	2.170
Number of reflections	16973	1672
<I/σ(I)>	22.71
Completeness [%]	99.8
Redundancy	7.7
CC(1/2)	0.983	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	0.3-0.6M magnesium formate, 15-20% polyethylene glycol 3350