8SMO
Crystal structure of the complex between truncated MLLE domain of PABPC1 and engineered superPAM2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-27 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 |
| Unit cell lengths | 108.275, 108.275, 75.866 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.110 - 3.000 |
| R-factor | 0.2594 |
| Rwork | 0.256 |
| R-free | 0.29160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.520 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 27587 | 535 |
| <I/σ(I)> | 17.1 | 0.9 |
| Completeness [%] | 99.9 | |
| Redundancy | 9.7 | 6.7 |
| CC(1/2) | 0.995 | 0.385 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.1 M citric acid pH 4.0, 1.6 M ammonium sulfate |
