8SMG
Structure of SPO1 phage Tad2 in complex with 1''-2' gcADPR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 193 |
Detector technology | PIXEL |
Collection date | 2022-12-16 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 108.064, 108.064, 72.357 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.290 - 2.100 |
R-factor | 0.2265 |
Rwork | 0.224 |
R-free | 0.24990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8sme |
RMSD bond length | 0.002 |
RMSD bond angle | 0.456 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.290 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rpim | 0.022 | 0.719 |
Number of reflections | 15065 | 1204 |
<I/σ(I)> | 17.3 | |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 27.5 | 26.8 |
CC(1/2) | 0.996 | 0.743 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | Tris-HCl, glycerol, ammonium sulfate |