8SM2

Crystal Structure of the macaque FcalphaRI bound to macaque IgA Fc.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 43 21 2
Unit cell lengths	160.047, 160.047, 52.563
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.290 - 3.150
R-factor	0.2737
Rwork	0.273
R-free	0.29580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.261
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.200
High resolution limit [Å]	3.150	3.150
Rmerge	0.104	0.819
Rpim	0.046	0.542
Number of reflections	12135	481
<I/σ(I)>	25.7	0.7
Completeness [%]	98.0	81.9
Redundancy	5.8	3.9
CC(1/2)	0.990	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	294	100 mM ammonium sulfate 12% PEG 3350 100 mM Tris-HCl pH 8.5