8SLU
Crystal structure of human STEP (PTPN5) at cryogenic temperature (100 K) and high pressure (205 MPa)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE 7B2 |
Synchrotron site | CHESS |
Beamline | 7B2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-30 |
Detector | DECTRIS EIGER2 S 16M |
Wavelength(s) | 1.033 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.143, 63.466, 134.198 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.370 - 1.840 |
R-factor | 0.197 |
Rwork | 0.195 |
R-free | 0.23660 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.241 |
Data reduction software | DIMPLE (v2.6.1) |
Data scaling software | xia2 |
Phasing software | DIMPLE (v2.6.1) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.370 | 1.906 |
High resolution limit [Å] | 1.840 | 1.840 |
Rmerge | 0.114 | 2.127 |
Rmeas | 0.124 | 2.308 |
Rpim | 0.048 | 0.888 |
Number of reflections | 29880 | 2949 |
<I/σ(I)> | 10.71 | 0.64 |
Completeness [%] | 99.5 | 99.12 |
Redundancy | 6.6 | 6.6 |
CC(1/2) | 0.998 | 0.687 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.65 | 293.15 | 30% PEG 3350, 200 mM Li2SO4, 100 mM bis-tris pH 5.65 |