8SLC
High Affinity nanobodies against GFP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0750 |
| Spacegroup name | P 63 |
| Unit cell lengths | 149.435, 149.435, 127.013 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.910 - 2.970 |
| R-factor | 0.2208 |
| Rwork | 0.220 |
| R-free | 0.24140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.949 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.970 |
| High resolution limit [Å] | 2.910 | 7.320 | 2.910 |
| Rmerge | 0.117 | 0.043 | 0.647 |
| Total number of observations | 347306 | ||
| Number of reflections | 78984 | 3971 | 3919 |
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 99.9 | 100 | 100 |
| Redundancy | 4.4 | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M sodium acetate pH 4.6, 2 M sodium formate |
