8SLB
X-ray structure of CorA N-terminal domain in complex with conformation-specific synthetic antibody C12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-15 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 137.338, 44.527, 150.634 |
| Unit cell angles | 90.00, 108.01, 90.00 |
Refinement procedure
| Resolution | 45.820 - 2.040 |
| R-factor | 0.16826 |
| Rwork | 0.166 |
| R-free | 0.21785 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.817 |
| Data reduction software | XDS (20190417) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.820 | 2.090 |
| High resolution limit [Å] | 2.038 | 2.040 |
| Rmerge | 0.056 | 0.657 |
| Rmeas | 0.078 | 0.918 |
| Rpim | 0.054 | 0.637 |
| Number of reflections | 55678 | 4041 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 99.4 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | 8% dextrane sulfate, 17% PEG 3350, 0.02 M citric acid/0.08 M bis-Tris propane pH 8.8 |
