8SK0
Crystal structure of EvdS6 decarboxylase in ligand bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-07-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.984, 76.405, 148.556 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.970 - 1.510 |
| R-factor | 0.1743 |
| Rwork | 0.173 |
| R-free | 0.19890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.889 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.073 | 0.604 |
| Number of reflections | 106406 | 5314 |
| <I/σ(I)> | 25.32 | 2.1 |
| Completeness [%] | 98.9 | 99.7 |
| Redundancy | 4 | |
| CC(1/2) | 0.996 | 0.725 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | Protein concentration= 9mg/ml, Reservoir solution= 2% v/v tacsimate pH 5.0, 100mM sodium citrate tribasic dihydrate pH 5.6, 16% w/v polyethylene glycol [PEG] 3350 |
