8SJT
[3T14+10] Self-assembling left-handed tensegrity triangle with 14 interjunction base pairs and a 10 bp linker with R3 symmetry
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-08 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.00718 |
| Spacegroup name | H 3 |
| Unit cell lengths | 212.243, 212.243, 164.272 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.660 - 9.380 |
| R-factor | 0.1795 |
| Rwork | 0.178 |
| R-free | 0.20290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5w6w |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.706 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 80.205 | 80.205 | 10.571 |
| High resolution limit [Å] | 9.380 | 19.137 | 9.380 |
| Number of reflections | 1416 | 203 | 201 |
| <I/σ(I)> | 7.3 | 19.7 | |
| Completeness [%] | 85.5 | 100 | |
| Redundancy | 10.1 | 8.8 | 10.2 |
| CC(1/2) | 0.999 | 0.999 | 0.038 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 100 mM MOPS, 1.25 M magnesium sulfate |
