8SJM

[3T12] Self-assembling left-handed tensegrity triangle with 12 interjunction base pairs and R3 symmetry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-12
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00743
Spacegroup name	H 3
Unit cell lengths	162.319, 162.319, 109.158
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.860 - 8.080
R-factor	0.1093
Rwork	0.108
R-free	0.14750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w6w
RMSD bond length	0.004
RMSD bond angle	0.678
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	86.216	86.216	9.621
High resolution limit [Å]	8.080	14.039	8.080
Number of reflections	858	216	216
<I/σ(I)>	5.7	14.2	1.1
Completeness [%]	82.7
Redundancy	6.8		7.6
CC(1/2)	0.998	0.998	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	100 mM MOPS, 1.25 M magnesium sulfate