8SIG
Crystal Structure of PRMT4 with Compound YD1-288
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 84.887, 84.887, 130.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.910 - 1.780 |
| R-factor | 0.18369 |
| Rwork | 0.183 |
| R-free | 0.19928 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.327 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.780 | 4.830 | 1.780 |
| Rmerge | 0.078 | 0.038 | 0.992 |
| Rmeas | 0.082 | 0.040 | 1.043 |
| Rpim | 0.025 | 0.013 | 0.321 |
| Total number of observations | 553802 | ||
| Number of reflections | 53004 | 2871 | 2633 |
| <I/σ(I)> | 7.6 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 10.4 | 9.8 | 10.5 |
| CC(1/2) | 0.998 | 0.999 | 0.777 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2 M Calcium chloride dihydrate, 0.1 M HEPES sodium pH 7.5, 28% v/v Polyethylene glycol 400 |
