8SH5

Crystal structure of 3'cap-independent translation enhancers (CITE) from Pea enation mosaic virus RNA 2 (PEMV2) with Fab BL3-6K170A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979180
Spacegroup name	P 1 21 1
Unit cell lengths	63.346, 65.460, 89.817
Unit cell angles	90.00, 110.29, 90.00

Refinement procedure

Resolution	59.420 - 2.750
R-factor	0.2554
Rwork	0.251
R-free	0.29660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.578
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.420	2.848
High resolution limit [Å]	2.750	2.750
Rmerge	0.191	0.572
Rmeas	0.219
Rpim	0.104
Number of reflections	18060	1760
<I/σ(I)>	14.45
Completeness [%]	97.3	97.01
Redundancy	3.8	3.7
CC(1/2)	0.979	0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	5 mM MgCl2, 50 mM HEPES pH 7.0, 25% PEG 550, 2% Benzamidine hydrochloride