8SGG

Crystal structure of Cy137D09, a monoclonal antibody isolated from macaques immunized with an Epstein-Barr virus glycoprotein 350 (gp350) nanoparticle vaccine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-18
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	79.794, 71.463, 86.451
Unit cell angles	90.00, 107.25, 90.00

Refinement procedure

Resolution	36.120 - 2.030
R-factor	0.1926
Rwork	0.191
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8sgn
RMSD bond length	0.006
RMSD bond angle	0.876
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.030	2.030
Rmeas	0.171	0.722
Number of reflections	51917	2870
<I/σ(I)>	9.4
Completeness [%]	85.7
Redundancy	2.7
CC(1/2)		0.642

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2M calcium acetate, 0.1M sodium cacodylate pH 6.5, 17.5% w/v PEG 8000