8SGD

Crystal Structure of CDC3(G) - CDC10(Delta 1-10) heterocomplex from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS SIRIUS BEAMLINE MANACA
Synchrotron site	LNLS SIRIUS
Beamline	MANACA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-31
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97718
Spacegroup name	P 21 21 21
Unit cell lengths	58.277, 154.271, 156.264
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.350 - 2.660
R-factor	0.2085
Rwork	0.206
R-free	0.25540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.502
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.820
High resolution limit [Å]	2.660	7.900	2.660
Rmerge	0.421	0.068	2.901
Rmeas	0.438	0.071	3.018
Number of reflections	40940	1742	6435
<I/σ(I)>	8.23
Completeness [%]	99.2		98.5
Redundancy	10.03
CC(1/2)	0.995	0.998	0.471

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	27% polyethylene glycol 3,350, 100 mM BIS-TRIS pH 6,5 and 270 mM sodium iodide