8SFX
High Affinity nanobodies against GFP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-12 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 41 |
| Unit cell lengths | 111.122, 111.122, 194.203 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.670 - 1.950 |
| R-factor | 0.2106 |
| Rwork | 0.210 |
| R-free | 0.22750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.107 |
| Data reduction software | MOSFLM (6.2.3) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.130 | 1.403 |
| Number of reflections | 85311 | 12451 |
| <I/σ(I)> | 11.1 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1 M bis-tris propane pH 7.0, 1.2 M DL-malic acid pH 7.0 |
