8SE1

Structure of Full-length Human Protein Kinase C Beta 2 (PKCBII) in the Inactive Conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-20
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.979180
Spacegroup name	C 1 2 1
Unit cell lengths	130.556, 89.098, 160.714
Unit cell angles	90.00, 107.05, 90.00

Refinement procedure

Resolution	48.750 - 3.320
R-factor	0.2018
Rwork	0.199
R-free	0.25550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.605
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.470
High resolution limit [Å]	3.320	7.210	3.350
Rmerge	0.070	0.070	0.962
Rmeas	0.352	0.083
Rpim		0.044	0.582
Number of reflections	25781	2636	2603
<I/σ(I)>	16
Completeness [%]	99.3	98.4	99.9
Redundancy	3.5	3.4	3.7
CC(1/2)	0.906	0.994	0.464

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277.15	PEG8000, MES buffer, magnesium chloride