8SDW

Crystal structure of the non-myristoylated mutant [L8K]Arf1 in complex with a GDP analogue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	41.654, 55.015, 78.472
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.240 - 1.750
Rwork	0.190
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20rc3-4406)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.780
High resolution limit [Å]	1.750	4.750	1.750
Rpim	0.025
Number of reflections	18613	1035	819
<I/σ(I)>	27.1
Completeness [%]	98.7
Redundancy	6.2
CC(1/2)	0.996	0.997	0.976

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	277.15	0.2 M Sodium nitrate, 20% w/v Polyethylene glycol 3,350, pH 6.8