8SCA

Rec3 Domain from S. pyogenes Cas9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	53.360, 54.631, 73.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.170 - 1.670
R-factor	0.1874
Rwork	0.186
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.818
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.910	1.700
High resolution limit [Å]	1.670	1.670
Number of reflections	25725	1277
<I/σ(I)>	8.3
Completeness [%]	100.0
Redundancy	13.2
CC(1/2)	0.994	0.871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Bis-Tris pH 5.5, 0.2 M sodium chloride, 25% PEG3350