8SBL

Structure of HLA-A*24:02 in complex with peptide, LYLPVRVLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-27
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920
Spacegroup name	P 1 21 1
Unit cell lengths	109.512, 76.193, 111.779
Unit cell angles	90.00, 109.76, 90.00

Refinement procedure

Resolution	105.190 - 3.000
R-factor	0.1976
Rwork	0.193
R-free	0.26950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.177
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	105.190	3.210
High resolution limit [Å]	3.000	3.050
Rmerge	0.388	1.203
Rmeas	0.459	1.418
Rpim	0.244	0.746
Number of reflections	34967	4832
<I/σ(I)>	2.8	0.9
Completeness [%]	99.8	100
Redundancy	3.5	3.5
CC(1/2)	0.877	0.419

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	0.05M MES monohydrate pH 6.5, 22.5% Polyethylene Glycol w/v 3350