8SBL
Structure of HLA-A*24:02 in complex with peptide, LYLPVRVLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-27 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.512, 76.193, 111.779 |
| Unit cell angles | 90.00, 109.76, 90.00 |
Refinement procedure
| Resolution | 105.190 - 3.000 |
| R-factor | 0.1976 |
| Rwork | 0.193 |
| R-free | 0.26950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.177 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 105.190 | 3.210 |
| High resolution limit [Å] | 3.000 | 3.050 |
| Rmerge | 0.388 | 1.203 |
| Rmeas | 0.459 | 1.418 |
| Rpim | 0.244 | 0.746 |
| Number of reflections | 34967 | 4832 |
| <I/σ(I)> | 2.8 | 0.9 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.877 | 0.419 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.05M MES monohydrate pH 6.5, 22.5% Polyethylene Glycol w/v 3350 |
