8S9M
DNA cytosine-N4 methyltransferase (residues 79-324) from the Bdelloid rotifer Adineta vaga
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 57.581, 69.096, 124.498 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.680 - 1.490 |
R-factor | 0.1617 |
Rwork | 0.160 |
R-free | 0.19710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.783 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.680 | 1.540 |
High resolution limit [Å] | 1.490 | 1.490 |
Rpim | 0.084 | 0.780 |
Number of reflections | 40906 | 4026 |
<I/σ(I)> | 9.9 | 2.2 |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 10.3 | 4.9 |
CC(1/2) | 0.985 | 0.340 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.2 M Potassium citrate tribasic monohydrate, 20% PEG 3350 |