8S9L
Structure of monomeric FAM111A SPD V347D Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-18 |
Detector | DECTRIS EIGER2 S 16M |
Wavelength(s) | 0.979180 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.462, 70.031, 128.021 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.960 - 1.850 |
R-factor | 0.2194 |
Rwork | 0.218 |
R-free | 0.25240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8s9k |
RMSD bond length | 0.012 |
RMSD bond angle | 1.097 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 70.000 | 70.000 | 1.920 |
High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
Rmerge | 0.056 | 0.034 | 1.651 |
Rmeas | 0.061 | 0.037 | 1.786 |
Rpim | 0.024 | 0.015 | 0.675 |
Total number of observations | 352758 | ||
Number of reflections | 53268 | 5636 | 5228 |
<I/σ(I)> | 10.6 | ||
Completeness [%] | 99.8 | 99.5 | 100 |
Redundancy | 6.6 | 6.3 | 6.9 |
CC(1/2) | 0.997 | 0.999 | 0.509 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 100 mM Bis-Tris, 2 M ammonium sulfate |