8S9K
Structure of dimeric FAM111A SPD S541A Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.814, 83.765, 191.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.500 - 2.720 |
| R-factor | 0.1956 |
| Rwork | 0.193 |
| R-free | 0.24950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.516 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
| Rmerge | 0.241 | 0.104 | 1.268 |
| Rmeas | 0.261 | 0.114 | 1.368 |
| Rpim | 0.099 | 0.044 | 0.507 |
| Total number of observations | 222382 | ||
| Number of reflections | 32772 | 3497 | 3223 |
| <I/σ(I)> | 3.7 | ||
| Completeness [%] | 99.8 | 99.7 | 100 |
| Redundancy | 6.8 | 6.4 | 7.1 |
| CC(1/2) | 0.978 | 0.991 | 0.523 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20% w/v PEG8000, 100 mM Tris, 200 mM magnesium chloride |
