8S96
RNase A-Adenosine 5'-Heptaphosphate (RNaseA.p7A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2023-02-13 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.544178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.383, 31.347, 68.962 |
| Unit cell angles | 90.00, 90.67, 90.00 |
Refinement procedure
| Resolution | 34.480 - 1.680 |
| R-factor | 0.2124 |
| Rwork | 0.211 |
| R-free | 0.23670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1afu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.026 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHENIX (1.20.1_4487) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.480 | 1.780 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rpim | 0.029 | 0.396 |
| Number of reflections | 24657 | 3862 |
| <I/σ(I)> | 3.55 | |
| Completeness [%] | 99.9 | |
| Redundancy | 10.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 25% PEG4000, 20 mM citrate, pH 6, crystal soaked in 50 mM ligand, 30% PEG4000, 25% glycerol, 20 mM citrate |
