8S8Y
OPR3 variant R283E in its dimeric form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-08 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.8856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.227, 90.357, 78.128 |
| Unit cell angles | 90.00, 107.97, 90.00 |
Refinement procedure
| Resolution | 44.800 - 1.800 |
| Rwork | 0.187 |
| R-free | 0.21960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.800 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rpim | 0.080 | 1.410 |
| Number of reflections | 137014 | 2424 |
| <I/σ(I)> | 5.72 | |
| Completeness [%] | 97.6 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.994 | 0.150 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES/Tris (6.5), 50 mM ammonium sulfate, 8-16% PEG8000 |
