8S8T

KOD-H4 DNA polymerase mutant in a binary complex with DNA/DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000010
Spacegroup name	P 21 21 2
Unit cell lengths	111.552, 149.297, 66.216
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.450 - 3.000
R-factor	0.1873
Rwork	0.185
R-free	0.24240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.629
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.520	2.390
High resolution limit [Å]	2.250	2.250
Number of reflections	100438	16062
<I/σ(I)>	3.95
Completeness [%]	99.1
Redundancy	3.17
CC(1/2)	0.989	0.115

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	10% (w/v) Polyethylene glycol 8,000, 100 mM TRIS; pH 8.0 10% (v/v) Glycerol, 1 mM Tris(2-carboxyethyl)phosphine hydrochloride