8S79

Lotus japonicus NFR5 intracellular domain in complex with Nanobody 200

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9184
Spacegroup name	P 31 2 1
Unit cell lengths	126.755, 126.755, 137.875
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.490 - 2.290
R-factor	0.1976
Rwork	0.196
R-free	0.23050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.673
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.660	2.580
High resolution limit [Å]	2.290	2.290
Rmeas	0.091	1.486
Number of reflections	32844	1642
<I/σ(I)>	16.8	7.11
Completeness [%]	56.4
Redundancy	9.8	9
CC(1/2)	0.999	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292.15	0.1 M sodium cacodylate pH 6.5, 1.2 M sodium acetate