8S4T
DNA bound structure of PrgE from plasmid pCF10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-04-27 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.8856 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.776, 90.043, 131.848 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.740 - 2.670 |
R-factor | 0.23052 |
Rwork | 0.229 |
R-free | 0.25257 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.683 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.20.1_4487) |
Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.740 | 2.765 |
High resolution limit [Å] | 2.670 | 2.670 |
Rmeas | 0.116 | 2.067 |
Number of reflections | 26266 | 2536 |
<I/σ(I)> | 10.71 | 0.95 |
Completeness [%] | 99.8 | 99.06 |
Redundancy | 6.7 | 7 |
CC(1/2) | 0.998 | 0.628 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 15 % Polyethylene glycol 400, 50 mM MES pH 6.5, 80 mM Magnesium acetate, 15 mM Magnesium chloride |