8S13

apo c-KIT-wt kinase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-31
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9999
Spacegroup name	C 1 2 1
Unit cell lengths	99.280, 69.320, 56.130
Unit cell angles	90.00, 117.72, 90.00

Refinement procedure

Resolution	44.860 - 2.000
R-factor	0.206
Rwork	0.204
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.602
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.860	2.100
High resolution limit [Å]	2.000	2.000
Number of reflections	22876	3112
<I/σ(I)>	14.2
Completeness [%]	99.6
Redundancy	6.9
CC(1/2)	0.998	0.686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277.15	protein concentration: 6.6 mg/mL, 16% PEG8000, 20% ethylene glycole, 100 mM bicine, 30 mM NaI, 30 mM NaBr, 30 mM NaF